PCR amplification of crude microbial DNA extracted from soil
نویسندگان
چکیده
منابع مشابه
Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples
Potent PCR inhibitors in blood and soil samples can cause false negative results from PCR-based clinical and forensic tests. We show that the effect of these inhibitors is primarily upon Taq DNA polymerase, since mutational alteration of the polymerase can overcome the inhibition to the extent that no DNA purification is now required. An N-terminal deletion (Klentaq1) is some 10-100-fold inhibi...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 1997
ISSN: 0266-8254,1472-765X
DOI: 10.1046/j.1472-765x.1997.00232.x